Journal: Cell reports

Article Title: Genetic- and diet-induced ω -3 fatty acid enrichment enhances TRPV4-mediated vasodilation in mice

doi: 10.1016/j.celrep.2022.111306

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Endothelial Cell Growth Medium MV 2 , Promo Cell , C-22121.

Techniques: Recombinant, Protease Inhibitor, Staining, Western Blot, Membrane, Protein Extraction, Plasmid Preparation, Software, Imaging, Microscopy

Journal: Respiratory Research

Article Title: The Tie-2 agonist PMC-403 inhibits endothelial damage induced by e-cigarette vapor or LPS

doi: 10.1186/s12931-026-03928-4

Figure Lengend Snippet: The cartridge of the e-cigarette was filled with either base or nicotine. The vape was caught by tubes and directed to a falcon filled with endothelial growth medium. The closed system was attached to a smoke trapping bottle which was ultimately attached to a vacuum pump, thereby imitating a standardized vaping process. The resulting medium was then filtered through a 0.20 µm filter, considered 100% and used for further experiments. Created in BioRender. Stahl, K. (2026) https://BioRender.com/jepgrde . Abbreviations: Angpt-2 – Angiopoietin-2, ECVE – e-cigarette vapor extract, HUVEC – Human umbilical vein endothelial cells

Article Snippet: As an additional endothelial cell type, primary human pulmonary microvascular endothelial cells (HPMECs; PromoCell, Heidelberg, Germany; Cat. No. C-12281) were used and cultured in endothelial cell growth medium MV2 (PromoCell; Cat. No. C-22022).

Techniques:

Journal: Respiratory Research

Article Title: The Tie-2 agonist PMC-403 inhibits endothelial damage induced by e-cigarette vapor or LPS

doi: 10.1186/s12931-026-03928-4

Figure Lengend Snippet: HUVECs were stimulated with either ECVE base ( n =4), ECVE nic at different dilutions ( n =3 for 50%, n =5 for 100%) or control ( n =4 in A, n =3 in B) for 24 hours and ( A ) Angpt-2 concentrations in the supernatant were measured by ELISA. B Additionally, Angpt-2 mRNA transcription was measured by qPCR in HUVECs after stimulation for 24 hours. Results are shown as dot plots with mean ± SD. One-way ANOVA with Tukey’s post-hoc test was performed. P -values < 0.05 are considered significant. Abbreviations: Angpt-2 - Angiopoietin-2, ECVE – e-cigarette vapor extract, HUVECs – human umbilical vein endothelial cells, SD – standard deviation

Article Snippet: As an additional endothelial cell type, primary human pulmonary microvascular endothelial cells (HPMECs; PromoCell, Heidelberg, Germany; Cat. No. C-12281) were used and cultured in endothelial cell growth medium MV2 (PromoCell; Cat. No. C-22022).

Techniques: Control, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Respiratory Research

Article Title: The Tie-2 agonist PMC-403 inhibits endothelial damage induced by e-cigarette vapor or LPS

doi: 10.1186/s12931-026-03928-4

Figure Lengend Snippet: A HUVECs were stimulated with ECVE nic or control (EBM-2) for 12 hours (incubation). Then, 100 ng/ml LPS with co-factors LBP and CD14 or co-factors alone (sham) were added and incubated for another 18 hours. Created in BioRender. Stahl, K. (2026) https://BioRender.com/nu4iwxz . B Angpt-2 concentrations in the supernatant were measured by ELISA when LPS was added as well as 12 and 18 hours after LPS or sham stimulation and are shown over time. Each group consisted of n =6. Mixed ANOVA with according post-tests were performed. C 18 hours after stimulation with LPS or sham Angpt-2 mRNA transcription was measured by qPCR. Each group consisted of n =9. D Additionally, the Angpt-2/Tie2 mRNA ratio is shown after initial ECVE nic incubation and 18 hours of LPS or sham stimulation. ECVE nic / sham consisted of n=8, all other groups consisted of n =9. Groups were analyzed by Kruskal-Wallis test with post-hoc Dunn test. E TER was measured at different time points by an ECIS system. HUVECs were pre-incubated with ECVE nic ( n =7) or control ( n =8) for 12 hours. Then half of the medium was changed and cells were stimulated with LPS. Results are shown as dot plots with mean ± SD. A linear mixed-effects model was used for analysis. P -values < 0.05 are considered significant. Abbreviations: Angpt-2 - Angiopoietin-2, ECIS - Electric cell-substrate impedance sensing, ECVE – e-cigarette vapor extract, HUVECs – human umbilical vein endothelial cells, LPS - lipopolysaccharide, SD – standard deviation, TER – transendothelial electrical resistance

Article Snippet: As an additional endothelial cell type, primary human pulmonary microvascular endothelial cells (HPMECs; PromoCell, Heidelberg, Germany; Cat. No. C-12281) were used and cultured in endothelial cell growth medium MV2 (PromoCell; Cat. No. C-22022).

Techniques: Control, Incubation, Enzyme-linked Immunosorbent Assay, Analysis, Electric Cell-substrate Impedance Sensing, Standard Deviation

Journal: Respiratory Research

Article Title: The Tie-2 agonist PMC-403 inhibits endothelial damage induced by e-cigarette vapor or LPS

doi: 10.1186/s12931-026-03928-4

Figure Lengend Snippet: A A schematic setup is shown for stimulation with the Tie2 agonist PMC-403 and its effects on HUVECs and HPMECs, accordingly. Created in BioRender. Stahl, K. (2026) https://BioRender.com/yc7s3fj . B HUVECs were stimulated with different concentrations of the Tie2 agonist PMC-403 or 500 ng/ml Angpt-1 for 1 hour. A western blot was performed and pAKT and AKT were analyzed. C HPMECs were incubated with 1 µg/ml PMC-403 for 1 hour and then stimulated with LPS. TER was measured by ECIS over time and group differences over time were analyzed by a linear mixed-effects model accordingly. D Group differences are shown 6.5 hours after LPS stimulation. Results are shown as dot plots with mean ± SD and each group consisted of n =4. P -values < 0.05 are considered significant. Abbreviations: Angpt-1 - Angiopoietin-1, ECIS - Electric cell-substrate impedance sensing, HPMECs - human pulmonary microvascular endothelial cells, HUVECs – human umbilical vein endothelial cells, LPS - lipopolysaccharide, SD – standard deviation, TER – transendothelial electrical resistance

Article Snippet: As an additional endothelial cell type, primary human pulmonary microvascular endothelial cells (HPMECs; PromoCell, Heidelberg, Germany; Cat. No. C-12281) were used and cultured in endothelial cell growth medium MV2 (PromoCell; Cat. No. C-22022).

Techniques: Western Blot, Incubation, Electric Cell-substrate Impedance Sensing, Standard Deviation